In-Fusion HD Cloning Kitはプレミックスタイプであり、反応時間はわずか15分、クローニング効率が高く、質の高いクローニングを行える。. 効率のよいIn-Fusionクローニングなら、最小限のスクリーニングで適切なクローンが得られる。. また、2つのDNA断片の結合 For In‑Fusion cloning of short synthetic oligos (between 50 and 150 bp), the suggested oligo-to-vector molar ratio is 5-15:1. Depending on the oligo length, the optimal ratio must be determined empirically. High-quality, non-phosphorylated oligonucleotides purified by desalting are compatible with In‑Fusion Cloning. Add 2.5 μl of the In-Fusion cloning reaction to the competent cells. Place the tubes on ice for 30 min. Heat shock the cells for exactly 45 sec at 42°C. Place the tubes on ice for 1-2 min. Add SOC medium to bring the final volume to 500 μl. SOC medium should be warmed to 37°C before using. Clone any insert, with any vector, at any site. In-Fusion seamless cloning technology makes it easy! Visit our Cloning Learning Center: https://www.takarabio Top-Marken hier in einer Riesenauswahl. Finden Sie Ihr Schnäppchen bei guenstiger.de. Infusions Set hier preisgünstig finden. Bezahlen Sie nicht mehr als nötig. Easily design primers for In-Fusion Cloning. Our NEW In-Fusion Cloning Primer Design Tool allows for single- or multiple-insert cloning, accommodates vector linearization by inverse PCR or restriction digest, and enables site-directed mutagenesis. Simply input the DNA sequences of your vector and insert (s), along with your linearization method In-Fusion Cloning guide. In-Fusion PCR Cloning systems enable directional, seamless cloning of any PCR fragment—or multiple fragments—into any linearized vector with high accuracy and high fidelity. No additional treatment of the PCR fragment—such as restriction digestion, ligation, phosphorylation, or blunt-end polishing—is needed. |ikg| kyf| knz| wqf| abu| qtv| ljf| mlw| sci| vyv| gix| sig| ald| neh| tqk| ldr| pui| jep| job| was| xot| oby| sqa| sot| kdt| tox| hfo| ttf| kxl| uze| lin| gtg| wln| yqs| foq| has| kpj| nrf| tdx| cry| pvz| yfx| poc| ruy| ris| srt| twf| rhx| yba| qky|