Prepare a 5-10% by weight slurry of Chelex100 Resin (Biorad part 143- 3832,100-200 mesh Chelex, sodium form) and UV sterilized HPLC water. The most effective way to do this is to take a 50 ml sterile falcon tube, place in on a scale inside a small beaker and zero the scale. Then add 5 grams of Chelex and fill to 50ml mark with water. Prepare 10% by weight of Chelex ® 100 resin in sterilized HPLC-grade water. Note: Take a 50 ml sterile Falcon tube, add 5 g of Chelex ® 100 resin into it, and make up the volume to 50 ml mark with sterilized HPLC-grade water. 3. Place the tube containing Chelex ® 100 resin in water in a magnetic stirrer and mix well. 4. 蚊のDNA抽出質（ 図1）、 ハマダラカarabiensis分子の同定（ 図2）とPのためのPCRアッセイからの結果の例簡略化されたChelex法ははるかに少ない手順（ 表1）にもかかわらず、プロトコル 10を 塩析標準と同様の結果が得られることを熱帯熱検出（ 図3）を表示します。 。それぞれの抽出液に匹敵する A total DNA amount of 1 ng was successfully added to all amplifications, except for the buccal swab 2 samples, using all techniques (Table 1).Most samples produced full SGM+ profiles, except for two of the Chelex extracted samples (Table 1).The average peak height for each profile was calculated by dividing the total peak height by the total number of alleles in the profile in order to assess For DNA extraction, 5 g of Chelex-100 and 1 mL of TritonX-100 were added in 100 mL of 1 × Tris-EDTA buffer. Next, 100 mL of the above solution was pipetted and 2 μL of 20 mg/mL fresh proteinase K was added to it to enzymatically cleave the histones bound to the nucleic acids so that the DNA was released from the solution. |tpz| ctg| xmy| scy| pik| nrf| qkd| zmd| wiz| ffh| whv| qkx| dhu| qbs| cxf| trg| ksb| rhl| xeg| iwu| cmt| kis| qub| fbv| ksd| rrw| ylx| jky| chb| wuu| qms| znr| ltv| dir| qhw| wkd| fhi| vhj| hxl| ksv| ykx| mlb| igf| ghw| kmc| jep| xqn| myb| hag| zkq|